Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22119740.
- Also identified by DOI 10.1038/nbt.2052 and PMC identifier 3357310.
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Abstract
We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.
Medical subject headings
- Cell Differentiation
- Cell Lineage
- DNA Methylation
- Epigenesis, Genetic
- Fetal Blood
- Induced Pluripotent Stem Cells
- Keratinocytes