A new concept in treatment of burn injury: controlled slow-release granulocyte-monocyte colony-stimulating factor chitosan gel system.

Abstract

The aim of the study is to investigate the effectiveness of the controlled slow-release granulocyte-monocyte colony-stimulating factor (GM-CSF) system in burn wound healing. In vivo effect of controlled slow-release GM-CSF from chitosan gel on burn wound healing was evaluated on 18 Wistar-Albino rats, weighing between 250 and 300 g. They were randomly divided into 3 groups; (1) burned only group (n = 6), (2) burned + chitosan group (n = 6), (3) burned + chitosan + GM-CSF group (n = 6). Wound area was measured macroscopically. Hematoxylin and eosin and Masson's trichrome stained sections were evaluated for wound healing and tissue response to the polymer. The best healing process was observed with the controlled slow-release GM-CSF-applied group (group 3) in which the wound area was significantly narrowed. The study demonstrated the positive contribution of the single-dose controlled slow-release GM-CSF from chitosan gel on burn wound healing.