Controlled gene expression in primary Lgr5 organoid cultures.
basic_science · Level V
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- Record sourced from PubMed, PMID 22138822.
- Also identified by DOI 10.1038/nmeth.1802.
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Abstract
The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.
Medical subject headings
- Intestinal Mucosa
- Organoids
- Receptors, G-Protein-Coupled