Fabrication of duplex DNA microarrays incorporating methyl-5-cytosine.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22139143.
- Also identified by DOI 10.1039/c1lc20698b and PMC identifier 4205155.
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Abstract
We synthesized customized double-stranded DNA microarrays including methyl-5-cytosine at CpG dinucleotides and produced all 163,555 possible 8-mers (un-, hemi-, and di-methylated) to gain insight into how methylation affects transcription factor binding. An antibody to methyl-5-cytidine showed greater binding to the methylated DNA, demonstrating efficient incorporation of methyl-5-cytosine into the synthesized DNA. In contrast, binding of the transcription factor CREB was inhibited by CpG methylation. This platform represents a powerful new technology to evaluate the effect of DNA methylation on protein binding in any sequence context.
Medical subject headings
- 5-Methylcytosine
- DNA
- Oligonucleotide Array Sequence Analysis