Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22146948.
- Also identified by DOI 10.1039/c1lc20879a.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we report a wound-healing benefit induced by addition of HGF to the cell culture medium (19.2 vs. 13.5 μm h(-1) healing rate).
Medical subject headings
- Epithelium
- Hepatocyte Growth Factor
- Microfluidic Analytical Techniques
- Microfluidics
- Wound Healing