B-SOLANA: an approach for the analysis of two-base encoding bisulfite sequencing data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22155865.
- Also identified by DOI 10.1093/bioinformatics/btr660 and PMC identifier 3268236.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Bisulfite sequencing, a combination of bisulfite treatment and high-throughput sequencing, has proved to be a valuable method for measuring DNA methylation at single base resolution. Here, we present B-SOLANA, an approach for the analysis of two-base encoding (colorspace) bisulfite sequencing data on the SOLiD platform of Life Technologies. It includes the alignment of bisulfite sequences and the determination of methylation levels in CpG as well as non-CpG sequence contexts. B-SOLANA enables a fast and accurate analysis of large raw sequence datasets. The source code, released under the GNU GPLv3 licence, is freely available at http://code.google.com/p/bsolana/. b.kreck@ikmb.uni-kiel.de Supplementary data are available at Bioinformatics online.
Medical subject headings
- DNA Methylation
- Sequence Analysis, DNA
- Software