Microfluidic capture and release of bacteria in a conical nanopore array.

Guo, Peng; Hall, Eric W; Schirhagl, Romana; Mukaibo, Hitomi; Martin, Charles R; Zare, Richard N · Lab Chip · 2012

basic_science · Level V

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Abstract

We present a simple and inexpensive method for the capture and release of bacteria contained in an array of conical nanopores on a membrane inside a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a defined orientation with over 500 bacteria per membrane with viabilities as high as 100%. The device can also specifically capture cyanobacteria from a mixed suspension of cyanobacteria and chlamydomonas with a selectivity as high as 90%.

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