Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22177619.
- Also identified by DOI 10.1016/j.biomaterials.2011.11.062.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A femtosecond laser based transfection method using off-resonance plasmonic gold nanoparticles is described. For human cancer melanoma cells, the treatment leads to a very high perforation rate of 70%, transfection efficiency three times higher than for conventional lipofection, and very low toxicity (<1%). Off-resonance laser excitation inhibited the fracture of the nanoparticles into possibly toxic DNA intercalating particles. This efficient and low toxicity method is a promising alternative to viral transfection for skin cancer treatment.
Medical subject headings
- Genetic Therapy
- Lasers
- Skin Neoplasms
- Transfection