Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22179619.
- Also identified by DOI 10.1039/c2lc21058d.
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Abstract
We have developed a method to analyze the substrate transport of ATP-binding cassette (ABC) transporters, which are associated with drug resistance in tumor cells. Our microfluidic method is well suited to the single-vesicle estimation of substrate transport and the rapid drug screening of ABC transporters. Using this method, we have demonstrated, for the first time, the analysis of substrate transport by a single transporter and performed drug-inhibition experiments in less than 3 h.
Medical subject headings
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Drug Resistance, Neoplasm
- Drug Screening Assays, Antitumor
- Microfluidic Analytical Techniques
- Neoplasms