Label-free Raman observation of cytochrome c dynamics during apoptosis.
basic_science · Level V
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- Record sourced from PubMed, PMID 22184220.
- Also identified by DOI 10.1073/pnas.1107524108 and PMC identifier 3252932.
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Abstract
We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.
Medical subject headings
- Apoptosis
- Cytochromes c
- Spectrum Analysis, Raman
- Staining and Labeling