Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
basic_science · Level V
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- Record sourced from PubMed, PMID 22194958.
- Also identified by DOI 10.1371/journal.pone.0028954 and PMC identifier PMC2620946.
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Abstract
A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.