Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22206011.
- Also identified by DOI 10.1371/journal.pone.0029352 and PMC identifier 3244249.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
Medical subject headings
- Animals
- Antibodies, Monoclonal
- Antibodies, Monoclonal/immunology
- Antigens
- Antigens/analysis
- Antigens/chemistry
- Antigens/immunology
- Brain
- Brain/metabolism
- Drosophila Proteins
- Drosophila Proteins/analysis
- Drosophila Proteins/chemistry
- Drosophila Proteins/immunology
- Drosophila melanogaster
- Drosophila melanogaster/cytology
- Drosophila melanogaster/metabolism
- Electrophoresis, Polyacrylamide Gel
- Hybridomas
- Immunoprecipitation
- Intracellular Space
- Intracellular Space/metabolism
- Mass Spectrometry
- Nerve Tissue Proteins
- Nerve Tissue Proteins/analysis
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/immunology
- Protein Structure, Tertiary
- Protein Transport
- Staining and Labeling