A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22216131.
- Also identified by DOI 10.1371/journal.pone.0028879 and PMC identifier 3244418.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.
Medical subject headings
- Biopsy
- Viruses