Characterization of fluorescent eye markers for mammalian transgenic studies.
basic_science · Level V
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- Record sourced from PubMed, PMID 22216292.
- Also identified by DOI 10.1371/journal.pone.0029486 and PMC identifier PMC2668200.
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Abstract
Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.