Blotting protein complexes from native gels to electron microscopy grids.
basic_science · Level V
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- Record sourced from PubMed, PMID 22231640.
- Also identified by DOI 10.1038/nmeth.1840.
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Abstract
We report a simple and generic method for the direct transfer of protein complexes separated by native gel electrophoresis to electron microscopy grids. After transfer, sufficient material remains in the gel for identification and characterization by mass spectrometry. The method should facilitate higher-throughput single-particle analysis by substantially reducing the time needed for protein purification, as demonstrated for three complexes from Thermoplasma acidophilum.
Medical subject headings
- Microscopy, Electron