Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.
basic_science · Level V
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- Record sourced from PubMed, PMID 22231642.
- Also identified by DOI 10.1038/nmeth.1841 and PMC identifier 3304438.
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Abstract
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
Medical subject headings
- Actins
- Cytoskeleton