Serial two-photon tomography for automated ex vivo mouse brain imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22245809.
- Also identified by DOI 10.1038/nmeth.1854 and PMC identifier 3297424.
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Abstract
Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.
Medical subject headings
- Anatomy, Cross-Sectional
- Brain
- Image Interpretation, Computer-Assisted
- Microscopy, Fluorescence, Multiphoton
- Pattern Recognition, Automated
- Tomography