Negative effects of ADAMTS-7 and ADAMTS-12 on endplate cartilage differentiation.

Abstract

The roles of ADAMTS-7 and ADAMTS-12 in disc degeneration have not been previously examined. The purpose of this study was to examine the expression of ADAMTS-7 and ADAMTS-12 in the endplate cells isolated from patients with degenerative disc disease and to see whether they are associated with the pathological change of endplate. Sixty-four degenerated lumbar endplate specimens were obtained from the patients with degenerative disc disease categorized as type Modic I or II in magnetic resonance imaging (MRI) and 12 nondegenerative specimens as control (vertebra burst fracture patients without degenerative change in MRI) during surgical procedures. The expression of ADAMTS-7 and ADAMTS-12 was examined by real-time PCR and Western blotting. A statistically significant increase in mRNA expression of ADAMTS-7 and ADAMTS-12 was observed in the endplate cells in degenerative discs compared with nondegenerative discs. The corresponding protein levels of ADAMTS-7 and ADAMTS-12 had the same expression patterns. Moreover, ADAMTS-7 and ADAMTS-12 down-regulated the expression of Col II, Sox9, and Col X the marker genes for chondrogenesis. Our results indicate that ADAMTS-7 and ADAMTS-12 appear to be potent negative regulators of endplate cartilage development.