An artery-specific fluorescent dye for studying neurovascular coupling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22266543.
- Also identified by DOI 10.1038/nmeth.1857 and PMC identifier 3392962.
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Abstract
We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.
Medical subject headings
- Brain
- Cerebral Arteries
- Fluorescent Dyes
- Microscopy, Fluorescence, Multiphoton
- Neurons
- Voltage-Sensitive Dye Imaging