In vivo protein crystallization opens new routes in structural biology.
basic_science · Level V
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- Record sourced from PubMed, PMID 22286384.
- Also identified by DOI 10.1038/nmeth.1859 and PMC identifier 3429599.
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Abstract
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Medical subject headings
- Crystallography
- Crystallography, X-Ray
- Proteins