Position-specific chemical modification and quantitative proteomics disclose protein orientation adsorbed on silica nanoparticles.
basic_science · Level V
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- Record sourced from PubMed, PMID 22296027.
- Also identified by DOI 10.1021/nl2044524.
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Abstract
We describe a method for determining the orientation of cytochrome c, RNase A, and lysozyme on silica nanoparticles (SNPs) using chemical modification combined with proteolysis-mass spectrometry. The proteins interacted with SNPs through preferential adsorption sites, which are dependent on SNP diameter; 4 nm SNPs induce greater structural stabilization than 15 nm particles, presumably due to greater surface curvature of the former. These results suggest that nanoparticle size and protein structure influence protein orientation on SNPs.
Medical subject headings
- Nanostructures
- Protein Interaction Mapping
- Proteins
- Silicon Dioxide