A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22305104.
- Also identified by DOI 10.1016/j.biomaterials.2012.01.012 and PMC identifier 4022101.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.
Medical subject headings
- Intestine, Small
- Intestines
- Regeneration
- Regenerative Medicine
- Tissue Scaffolds