Library-free methylation sequencing with bisulfite padlock probes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22306810.
- Also identified by DOI 10.1038/nmeth.1871 and PMC identifier 3461232.
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Abstract
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
Medical subject headings
- DNA
- DNA Probes
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Sulfites