Radial sizing of lipid nanotubes using membrane displacement analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22313341.
- Also identified by DOI 10.1021/nl203983e and PMC identifier 3303199.
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Abstract
We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40-56 nm, upon increasing cholesterol content from 0 to 20%.
Medical subject headings
- Fluorescence Recovery After Photobleaching
- Lipids
- Membranes, Artificial
- Nanotubes