Hydrodynamic shearing of DNA in a polymeric microfluidic device.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22314498.
- Also identified by DOI 10.1039/c2lc21122j.
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Abstract
With the advent of next-generation sequencing (NGS) systems and the associated high throughput they afford, the input to these machines requires manageable lengths of fragments (~1000 bp) produced from chromosomal DNAs. Therefore, it is critical to develop devices that can shear DNA in a controlled fashion. We report a polymer-based microfluidic device that establishes an efficient and inexpensive platform with performance comparable to a commercially available bench-top system.
Medical subject headings
- DNA
- Microfluidic Analytical Techniques