Application of click chemistry to the production of DNA microarrays.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22318451.
- Also identified by DOI 10.1039/c2lc21096g.
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Abstract
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
Medical subject headings
- Alkynes
- Azides
- Click Chemistry
- DNA
- Oligonucleotide Array Sequence Analysis