Spatiotemporal control of gene expression by a light-switchable transgene system.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22327833.
- Also identified by DOI 10.1038/nmeth.1892.
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Abstract
We developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. The transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. This transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.
Medical subject headings
- DNA
- Gene Expression
- Transgenes