Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22334354.
- Also identified by DOI 10.1039/c2lc21170j.
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Abstract
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.
Medical subject headings
- Microfluidic Analytical Techniques