Photothermal single-particle microscopy: detection of a nanolens.
basic_science · Level V
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- Record sourced from PubMed, PMID 22352758.
- Also identified by DOI 10.1021/nn300181h.
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Abstract
Combining quantitative photothermal microscopy and light scattering microscopy as well as accurate MIE scattering calculations on single gold nanoparticles, we reveal that the mechanism of photothermal single-molecule/particle detection is quantitatively explained by a nanolensing effect. The lensing action is the result of the long-range character of the refractive index profile. It splits the focal detection volume into two regions. Our results lay the foundation for future developments and quantitative applications of single-molecule absorption microscopy.
Medical subject headings
- Lenses
- Microscopy
- Nanotechnology
- Temperature