Electronic barcoding of a viral gene at the single-molecule level.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22352964.
- Also identified by DOI 10.1021/nl300372a and PMC identifier 3572535.
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Abstract
A new single-molecule approach for rapid and purely electronic discrimination among similar genes is presented. Combining solid-state nanopores and γ-modified synthetic peptide nucleic acid probes, we accurately barcode genes by counting the number of probes attached to each gene and measuring their relative spacing. We illustrate our method by sensing individual genes from two highly similar human immunodeficiency virus subtypes, demonstrating feasibility of a novel, single-molecule diagnostic platform for rapid pathogen classification.
Medical subject headings
- DNA Barcoding, Taxonomic
- Nanotechnology
- Viral Proteins