CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.
basic_science · Level V
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- Record sourced from PubMed, PMID 22357696.
- Also identified by DOI 10.1093/infdis/jis032 and PMC identifier 3295606.
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Abstract
We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.
Medical subject headings
- DNA, Viral
- DNA-Cytosine Methylases
- Herpesvirus 8, Human
- Lymphoma, Primary Effusion
- Sarcoma, Kaposi