Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22361780.
- Also identified by DOI 10.1039/c2lc21084c and PMC identifier 3965373.
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Abstract
We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells s(-1) with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s(-1) or 3000 beads s(-1). Fast switching (30 μs) and a small perturbation volume (~90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.
Medical subject headings
- Cell Separation
- Fluorescent Dyes
- Lasers
- Microfluidic Analytical Techniques