DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22375622.
- Also identified by DOI 10.1021/nl204058u and PMC identifier 3324635.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.
Medical subject headings
- DNA
- Endothelial Cells
- Nanotubes, Carbon
- Pinocytosis
- rac1 GTP-Binding Protein