Immunosensor towards low-cost, rapid diagnosis of tuberculosis.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 22395572.
- Also identified by DOI 10.1039/c2lc21131a.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A rapid, accurate tuberculosis diagnostic tool that is compatible with the needs of tuberculosis-endemic settings is a long-sought goal. An immunofluorescence microtip sensor is described that detects Mycobacterium tuberculosis complex cells in sputum in 25 minutes. Concentration mechanisms based on flow circulation and electric field are combined at different scales to concentrate target bacteria in 1 mL samples onto the surfaces of microscale tips. Specificity is conferred by genus-specific antibodies on the microtip surface. Immunofluorescence is then used to detect the captured cells on the microtip. The detection limit in sputum is 200 CFU mL(-1) with a success rate of 96%, which is comparable to PCR.
Medical subject headings
- Fluorescent Antibody Technique
- Microfluidic Analytical Techniques
- Mycobacterium tuberculosis
- Sputum
- Tuberculosis