Tracking protein aggregation and mislocalization in cells with flow cytometry.
basic_science · Level V
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- Record sourced from PubMed, PMID 22426490.
- Also identified by DOI 10.1038/nmeth.1930.
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Abstract
We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
Medical subject headings
- Flow Cytometry
- Huntington Disease
- Nerve Tissue Proteins