Two-color DNA nanoprobe of intracellular dynamics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22463480.
- Also identified by DOI 10.1021/nl300683p.
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Abstract
We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo.
Medical subject headings
- Color
- DNA Probes
- Nanotechnology