Direct visualization of specifically modified extracellular glycans in living animals.
basic_science · Level V
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- Record sourced from PubMed, PMID 22466794.
- Also identified by DOI 10.1038/nmeth.1945 and PMC identifier 3437987.
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Abstract
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
Medical subject headings
- Caenorhabditis elegans
- Green Fluorescent Proteins
- Heparan Sulfate
- Single-Chain Antibodies