Genome-scale promoter engineering by coselection MAGE.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22484848.
- Also identified by DOI 10.1038/nmeth.1971 and PMC identifier 3428217.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Multiplex automated genome engineering (MAGE) uses short oligonucleotides to scarlessly modify genomes; however, insertions >10 bases are still inefficient but can be improved substantially by selection of highly modified chromosomes. Here we describe 'coselection' MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.
Medical subject headings
- Genetic Engineering
- Promoter Regions, Genetic