An optimized two-finger archive for ZFN-mediated gene targeting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22543349.
- Also identified by DOI 10.1038/nmeth.1994 and PMC identifier 3443678.
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Abstract
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
Medical subject headings
- Gene Targeting
- Zinc Fingers