Automated whole-cell patch-clamp electrophysiology of neurons in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22561988.
- Also identified by DOI 10.1038/nmeth.1993 and PMC identifier 3427788.
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Abstract
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.
Medical subject headings
- Automation, Laboratory
- Neurons
- Patch-Clamp Techniques