Rational design of true monomeric and bright photoactivatable fluorescent proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22581370.
- Also identified by DOI 10.1038/nmeth.2021.
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Abstract
Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations and forms aggregates when labeling membrane proteins, limiting its application as a fusion partner. We solved the crystal structure of tetrameric mEos2 and rationally designed improved versions, mEos3.1 and mEos3.2, that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density.
Medical subject headings
- Green Fluorescent Proteins
- Luminescent Proteins
- Microscopy, Fluorescence
- Protein Engineering