M-Track: detecting short-lived protein-protein interactions in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22581371.
- Also identified by DOI 10.1038/nmeth.2017 and PMC identifier 3428623.
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Abstract
We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.
Medical subject headings
- Histone-Lysine N-Methyltransferase
- Protein Interaction Mapping