Material-driven differentiation of induced pluripotent stem cells in neuron growth factor-grafted poly(ε-caprolactone)-poly(β-hydroxybutyrate) scaffolds.

Abstract

The potential of constructs comprising induced pluripotent stem (iPS) cells and biopolymers can be high for neurological surgery practice, if the systematic activity of neuronal regeneration is clarified. This study shows a guided differentiation of iPS cells toward neurons in neuron growth factor (NGF)-grafted poly(ε-caprolactone) (PCL)-poly(β-hydroxybutyrate) (PHB) scaffolds. The porosity of PCL-PHB scaffolds enhanced with increasing the concentration of salt particles (porogen) and the weight percentage of PCL. An increase in the graft concentration of NGF elevated the atomic ratios of N/C and O/C on the surface of NGF-grafted PCL-PHB scaffolds. In addition, incorporating heparin and NGF promoted the adhesion and viability of iPS cells in constructs. When the weight percentage of PCL increased, the viability of iPS cells reduced; however, more PCL in constructs benefited the adhesion of iPS cells. Under the influence of heparin and NGF, a high weight percentage of PCL and a long inductive period improved iPS cells to differentiate into neuron-like cells carrying βIII tubulin and inhibited other differentiation(s). The material-driven differentiation in NGF-grafted PCL-PHB constructs can be promising in guiding iPS cells to produce neurons for nerve tissue engineering.