2b-RAD: a simple and flexible method for genome-wide genotyping.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22609625.
- Also identified by DOI 10.1038/nmeth.2023.
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Abstract
We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.
Medical subject headings
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Genome
- Genotyping Techniques