Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22622966.
- Also identified by DOI 10.1039/c2lc21285d.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Here, we report a unique method to quantify the effects of in vivo-like extracellular matrix (ECM) for guiding differentiation of neural stem cells (NSCs) in three-dimensional (3D) microenvironments using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully monitored and quantified differentiation of NSCs in small volume ECMs and found that differentiation of NSCs, especially those differentiating towards neuronal and oligodendrocytic lineages, is significantly enhanced by 3D microenvironments reconstituted in the microfluidic channels.
Medical subject headings
- Cell Differentiation
- Microfluidic Analytical Techniques
- Neural Stem Cells