Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22648706.
- Also identified by DOI 10.1039/c2lc40204a.
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Abstract
This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.
Medical subject headings
- Alkaline Phosphatase
- Bioreactors
- Enzymes, Immobilized
- Polycarboxylate Cement
- Polyethyleneimine