Fluorescence axial localization with nanometer accuracy and precision.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22703235.
- Also identified by DOI 10.1021/nl301542c.
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Abstract
We describe a new technique, standing wave axial nanometry (SWAN), to image the axial location of a single nanoscale fluorescent object with sub-nanometer accuracy and 3.7 nm precision. A standing wave, generated by positioning an atomic force microscope tip over a focused laser beam, is used to excite fluorescence; axial position is determined from the phase of the emission intensity. We use SWAN to measure the orientation of single DNA molecules of different lengths, grafted on surfaces with different functionalities.
Medical subject headings
- DNA
- Fluorescence
- Nanotechnology