Visualization and analysis of 3D microscopic images.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22719236.
- Also identified by DOI 10.1371/journal.pcbi.1002519 and PMC identifier 3375219.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy