Atomic force microscopy with nanoscale cantilevers resolves different structural conformations of the DNA double helix.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22731615.
- Also identified by DOI 10.1021/nl301857p.
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Abstract
Structural variability and flexibility are crucial factors for biomolecular function. Here we have reduced the invasiness and enhanced the spatial resolution of atomic force microscopy (AFM) to visualize, for the first time, different structural conformations of the two polynucleotide strands in the DNA double helix, for single molecules under near-physiological conditions. This is achieved by identifying and tracking the anomalous resonance behavior of nanoscale AFM cantilevers in the immediate vicinity of the sample.
Medical subject headings
- DNA
- Microscopy, Atomic Force
- Nanostructures