PurityEst: estimating purity of human tumor samples using next-generation sequencing data.

Su, Xiaoping; Zhang, Li; Zhang, Jianping; Meric-Bernstam, Funda; Weinstein, John N · Bioinformatics · 2012

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Abstract

We developed a novel algorithm, PurityEst, to infer the tumor purity level from the allelic differential representation of heterozygous loci with somatic mutations in a human tumor sample with a matched normal tissue using next-generation sequencing data. We applied our tool to a whole cancer genome sequencing datasets and demonstrated the accuracy of PurityEst compared with DNA copy number-based estimation. PurityEst has been implemented in PERL and is available at http://odin.mdacc.tmc.edu/~xsu1/PurityEst.html.

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